Antibiotics methods and protocols
- xi, 358 pages : illustrations (some color) ; 26 cm.
- Methods in molecular biology, 1520. 1064-3745 ; Springer protocols. .
- Methods in molecular biology (Clifton, N.J.) ; v. 1520. Springer protocols (Series) .
"Published In: United States, 22 November 2016 " --Distributor's website
Includes bibliographical references and index.
Antibiotics : precious goods in changing times / Mining bacterial genomes for secondary metabolite gene clusters / Production of antimicrobial compounds by fermentation / Structure elucidation of antibiotics by NMR spectroscopy / Computer-aided drug design methods / Cytotoxicity assays as predictors of the safety and efficacy of antimicrobial agents / Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds / Determination of bacterial membrane impairment by antimicrobial agents / Mass-sensitive biosensor systems to determine the membrane interaction of analytes / Measurement of cell membrane fluidity by Laurdan GP : fluorescence spectroscopy and microscopy / In vitro assays to identify antibiotics targeting DNA metabolism / Fluorescence-based real-time activity assays to identify RNase P inhibitors / Reporter gene-based screening for TPP riboswitch activators / Cell-based fluorescent screen to identify inhibitors of bacterial translation initiation / Bacterial histidine kinases : overexpression, purification, and inhibitor screen / Expression profiling of antibiotic-resistant bacteria obtained by laboratory evolution / Sample preparation for mass-spectrometry based absolute protein quantification in antibiotic stress research / Label-free quantitation of ribosomal proteins from Bacillus subtilis for antibiotic research / Functional metagenomics to study antibiotic resistance / Epidemiological surveillance and typing methods to track antibiotic resistant strains using high throughput sequencing / Peter Sass -- Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert -- Henrik Harms, Gabriele M. König, and Till F. Schäberle -- Georgios Daletos, Elena Ancheeva, Raha S. Orfali, Victor Wray, and Peter Proksch -- Wenbo Yu and Alexander D. MacKerell Jr. -- Alexander Zipperer and Dorothee Kretschmer -- Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard -- Miriam Wilmes and Hans-Georg Sahl -- Sebastian G. Hoss and Gerd Bendas -- Kathi Scheinpflug, Oxana Krylova, and Henrik Strahl -- Allan H. Pang, Sylvie Garneau-Tsodikova, and Oleg V. Tsodikov -- Yu Chen, Xin Liu, Nancy Wu, and Carol A. Fierke -- Christina E. Lünse and Günter Mayer -- Federica Briani -- Mike Gajdiss, Michael Türck, and Gabriele Bierbaum -- Shingo Suzuki, Takaaki Horinouchi, and Chikara Furusawa -- Florian Bonn, Sandra Maass, and Dörte Becher -- Sina Schäkermann, Pascal Prochnow, and Julia E. Bandow -- Manish Boolchandani, Sanket Patel, and Gautam Dantas -- Miguel Paulo Machado, Bruno Ribeiro-Gonçalves, Mickael Silva, Mário Ramirez, and João André Carriço.