000			05494cam a2200661 i 4500
999			_c141 _d141
001			18827273
003			OSt
005			20181025121911.0
008			151022t20162016nyua b 001 0deng c
010			_a 2015955553
016	7		_a101676381 _2DNLM
020			_a9781493931477 (alk. paper)
020			_a1493931474 (alk. paper)
020			_z9781493931484 (ebook)
035			_a(OCoLC)ocn913556989
040			_aNLM _beng _cINAYA MEDICAL COLLEGE _erda _dYDXCP _dBTCTA _dNUI _dIAL _dOCLCF _dOCLCO _dOCL _dDLC
041			_aENG
042			_apcc
050	0	0	_aQP623.5.S63 _bR525 2016
060	0	0	_aW1 _bME9616J v.1372 2016
060	1	0	_aQU 25
082	0	0	_a572.88 MZR _223
100			_9120 _aMedarova,Zdravka
245	0	0	_aRNA imaging : _bmethods and protocols
264		1	_aNew York : _bHumana Press, _c[2016]
264		4	_c©2016
300			_axi, 218 pages : _billustrations (some color) ; _c27 cm.
336			_atext _btxt _2rdacontent
337			_aunmediated _bn _2rdamedia
338			_avolume _bnc _2rdacarrier
490	1		_aMethods in molecular biology, _x1064-3745 ; _v1372
490	1		_aSpringer protocols
504			_aIncludes bibliographical references and index.
505	0	0	_tImaging functional nucleic acid delivery to skin / _rRoger L. Kaspar [and others] -- _tIn vivo magnetic resonance imaging of small interfering RNA nanodelivery to pancreatic islets / _rPing Wang and Anna Moore -- _tMagnetic resonance spectroscopy of siRNA-based cancer therapy / _rMarie-France Penet [and others] -- _tTargeted delivery with imaging assessment of siRNA expressing nanocassettes into cancer / _rWei Chen and Lily Yang -- _tAnalyses of tumor burden in vivo and metastasis ex vivo using luciferase-expressing cancer cells in an orthotopic mouse model of neuroblastoma / _rFrances L. Byrne, Joshua A. McCarroll, and Maria Kavallaris -- _tIndium-labeling of siRNA for small animal SPECT imaging / _rSteven Jones and Olivia Merkel -- _tImaging of electrotransferred siRNA / _rMuriel Golzio and Justin Teissié -- _tWhole-body scanning PCR, a tool for the visualization of the in vivo biodistribution pattern of endogenous and exogenous oligonucleotides in rodents / _rJulien A. Boos and Iwan Beuvink -- _tsiRNA nanoparticles for ultra-long gene silencing in vivo / _rSeung Koo Lee and Ching-Hsuan Tung -- _tSensing miRNA : signal amplification by cognate RISC for intracellular detection of miRNA in live cells / _rAmol Kavishwar and Zdravka Medarova -- _tMolecular beacon-based microRNA imaging during neurogenesis / _rJonghwan Lee and Soonhag Kim -- _tHypoxia-responsive copolymer for siRNA delivery / _rFederico Perche [and others] -- _tControlling RNA expression in cancer using iron oxide nanoparticles detectable by MRI and in vivo optical imaging / _rZdravka Medarova, Mustafa Balcioglu, and Mehmet V. Yigit -- _tMicrovesicles : isolation, characterization for in vitro and in vivo procedures / _rKarmele Valencia and Fernando Lecanda -- _tPositive bioluminescence imaging of microRNA expression in small animal models using an engineered genetic-switch expression system, RILES / _rPatrick Baril and Chantal Pichon -- _tMicroRNA imaging in combination with diagnostic ultrasound and bubble liposomes for microRNA delivery / _rYoko Endo-Takahashi [and others].
520			_aThis detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.
650		0	_aRNA.
650		0	_aSmall interfering RNA.
650		0	_aImaging systems in biology.
650		0	_aGene expression.
650		0	_aRadiology.
650		0	_aMedicine.
650	1	2	_aRNA Interference
650	1	2	_aMolecular Imaging _xmethods
650		7	_aGene expression. _2fast _0(OCoLC)fst00939613
650		7	_aImaging systems in biology. _2fast _0(OCoLC)fst00967624
650		7	_aMedicine. _2fast _0(OCoLC)fst01014893
650		7	_aRadiology. _2fast _0(OCoLC)fst01088271
650		7	_aRNA. _2fast _0(OCoLC)fst01086244
650		7	_aSmall interfering RNA. _2fast _0(OCoLC)fst01121445
655		2	_aLaboratory Manuals
700	1		_aMedarova, Zdravka, _eeditor. _9120
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1372. _x1064-3745
830		0	_aSpringer protocols (Series) _x1949-2448
906			_a7 _bcbc _cpccadap _d2 _eepcn _f20 _gy-gencatlg
942			_2ddc _cBK